Antibody was affinity purified using an epitope specific to EAP6 immobilized on solid support. The epitope recognized by A300-352A maps to a region between residue 400 and 450 of human Ubiquitin Protein Ligase E3A using the numbering given in entry NP_000453.2 (GeneID 7337). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
ANCR, AS, CTCL tumor antigen se37-2, E6-AP, E6AP ubiquitin-protein ligase, EPVE6AP, HECT-type ubiquitin transferase E3A, HPVE6A, human papilloma virus E6-associated protein, human papillomavirus E6-associated protein, oncogenic protein-associated protein
between 400 and 450
2 - 8°C
Detection of human and mouse E6AP by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded), HEK293T (T; 50 µg), and mouse NIH 3T3 (M; 50 µg) cells. Antibodies: Affinity purified rabbit anti-E6AP antibody A300-352A (lot A300-352A-2) used for WB at 0.4 µg/ml (A) and 1 µg/ml (B) and used for IP at 3 µg/mg lysate. E6AP was also immunoprecipitated by rabbit anti-E6AP antibody A300-351A, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A and B).