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Rabbit anti-FHL2 Antibody, Affinity Purified - A300-333A - 100 µg - Bethyl Laboratories, Inc. - antibodies

Rabbit anti-FHL2 Antibody, Affinity Purified
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Size: 100 µg
£302.00
GBP | Euro | USD
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Catalogue number:
A300-333A
Name:
Rabbit anti-FHL2 Antibody, Affinity Purified
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Antibody was affinity purified using an epitope specific to FHL2 immobilized on solid support. The epitope recognized by A300-333A maps to a region between residue 225 and the C-terminus (residue 279) of human Four and a Half LIM domains 2 using the numbering given in entry NP_963849.1 (GeneID 2274).Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.

Applications:

WB, IP, IHC

Product Type:
Antibodies
Alternative Names:
AAG11, aging-associated gene 11, down-regulated in rhabdomyosarcoma LIM protein, DRAL, FHL-2, four and a half LIM domains protein 2, LIM domain protein DRAL, skeletal muscle LIM-protein 3, SLIM3, SLIM-3
Species Reactivity:
Human, Mouse
Immunogen:
Between 225 and C-term
Storage Temp:
2 - 8°C
Antibody Host:
Rabbit
Antibody Isotype:
Whole IgG
Antibody Type:
Polyclonal
UNSPSC Code:
12352203
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Detection of human and mouse FHL2 by western blot. Samples: Whole cell lysate (50 µg) from HeLa, TCMK-1, and NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-FHL2 antibody A300-333A (lot A300-333A-4) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

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Detection of human FHL2 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-FHL2 antibody A300-333A (lot A300-333A-4) used for IP at 3 µg per reaction. FHL2 was also immunoprecipitated by rabbit anti-FHL2 antibody A300-332A. For blotting immunoprecipitated FHL2, A300-333A was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

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Detection of human FHL2 by immunohistochemistry. Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-FHL2 (Cat. No. A300-333A Lot 4) used at a dilution of 1:2000 (0.5µg/ml). Detection: DAB

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