Antibody was affinity purified using an epitope specific to FHL2 immobilized on solid support. The epitope recognized by A300-333A maps to a region between residue 225 and the C-terminus (residue 279) of human Four and a Half LIM domains 2 using the numbering given in entry NP_963849.1 (GeneID 2274). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
AAG11, aging-associated gene 11, down-regulated in rhabdomyosarcoma LIM protein, DRAL, FHL-2, four and a half LIM domains protein 2, LIM domain protein DRAL, skeletal muscle LIM-protein 3, SLIM3, SLIM-3
between 225 and C-term
2 - 8°C
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Detection of human and mouse FHL2 by western blot. Samples: Whole cell lysate (50 µg) from HeLa, TCMK-1, and NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-FHL2 antibody A300-333A (lot A300-333A-4) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Detection of human FHL2 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-FHL2 antibody A300-333A (lot A300-333A-4) used for IP at 3 µg per reaction. FHL2 was also immunoprecipitated by rabbit anti-FHL2 antibody A300-332A. For blotting immunoprecipitated FHL2, A300-333A was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.