Antibody was affinity purified using an epitope specific to RFC1 immobilized on solid support. The epitope recognized by A300-320A maps to a region between residue 1100 and the C-terminus (residue 1148) of human Replication Factor C subunit 1 using the numbering given in entry NP_002904.3 (GeneID 5981). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
A1, A1 140 kDa subunit, activator 1 140 kDa subunit, activator 1 large subunit, activator 1 subunit 1, DNA-binding protein PO-GA, MHC binding factor, beta, MHCBFB, PO-GA, RECC1, replication factor C (activator 1) 1, 145kDa, replication factor C 140 kDa su
between 1100 and C-term
2 - 8°C
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Detection of human RFC1 by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded) and HEK293T (T; 50 µg) cells. Antibodies: Affinity purified rabbit anti-RFC1 antibody A300-320A (lot A300-320A-2) used for WB at 0.04 µg/ml (A) and 0.4 µg/ml (B) and used for IP at 6 µg/mg lysate. RFC1 was also immunoprecipitated by a prior lot (lot A300-320A-1) of this antibody. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 30 seconds (B).