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Rabbit anti-RAP1 Antibody, Affinity Purified

Catalogue number:
A300-306A
Size:
100 µg
Product is available in:
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Antibody was generated using recombinant full length human RAP1 (Telomeric repeat binding factor 2 interacting protein 1) according to entry NP_061848.2 (GeneID 54386). Antibody was affinity purified using the protein immobilized on solid support. Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.

  • Applications: WB, IP, IHC
Product Type:

Antibodies

Alternative Names:

dopamine receptor-interacting protein 5, DRIP5, hRap1, RAP1, RAP1 homolog, repressor/activator protein 1 homolog, telomeric repeat-binding factor 2-interacting protein 1, TERF2-interacting telomeric protein 1, TRF2-interacting telomeric protein 1, TRF2-in

Species Reactivity:

Human

Immunogen:

Full length

Storage Temp:

2 - 8°C

Antibody Host:

Rabbit

Antibody Isotype:

Whole IgG

Antibody Type:

Polyclonal

UNSPSC Code:

12352203

Detection of human RAP1 by western blot.

Detection of human RAP1 by western blot. Samples: Whole cell lysate (15 µg) from HeLa and HEK293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-RAP1 antibody A300-306A (lot A300-306A-9) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Detection of human RAP1 by western blot

Detection of human RAP1 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-RAP1 antibody A300-306A (lot A300-306A-9) used for IP at 6 µg per reaction. RAP1 was also immunoprecipitated by a previous lot of this antibody (lot A300-306A-8) and rabbit anti-RAP1 antibody A303-532A For blotting immunoprecipitated RAP1, A300-306A was used at 0.4 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Detection of human RAP1 by immunohistoch

Detection of human RAP1 by immunohistochemistry. Samples: FFPE sections of human lung carcinoma. Antibody: Affinity purified rabbit anti-RAP1 (Cat. No. A300-306A Lot9) used at a dilution of 1:5,000 (0.2µg/ml). Detection: DAB