Antibody was generated using recombinant full length human RAP1 (Telomeric repeat binding factor 2 interacting protein 1) according to entry NP_061848.2 (GeneID 54386). Antibody was affinity purified using the protein immobilized on solid support. Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
dopamine receptor-interacting protein 5, DRIP5, hRap1, RAP1, RAP1 homolog, repressor/activator protein 1 homolog, telomeric repeat-binding factor 2-interacting protein 1, TERF2-interacting telomeric protein 1, TRF2-interacting telomeric protein 1, TRF2-in
2 - 8°C
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Detection of human RAP1 by western blot. Samples: Whole cell lysate (15 µg) from HeLa and HEK293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-RAP1 antibody A300-306A (lot A300-306A-9) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Detection of human RAP1 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-RAP1 antibody A300-306A (lot A300-306A-9) used for IP at 6 µg per reaction. RAP1 was also immunoprecipitated by a previous lot of this antibody (lot A300-306A-8) and rabbit anti-RAP1 antibody A303-532A For blotting immunoprecipitated RAP1, A300-306A was used at 0.4 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.