Antibody was affinity purified using an epitope specific to KIAA0082 immobilized on solid support. The epitope recognized by A300-303A maps to a region between residues 1 and 50 of human KIAA0082 using the numbering given in TrEMBL entry Q8N1G2 (GeneID 23070). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
Cap methyltransferase 1, cap1 2'O-ribose methyltransferase 1, cap-specific mRNA (nucleoside-2'-O-)-methyltransferase 1, FtsJ methyltransferase domain containing 2, ftsJ methyltransferase domain-containing protein 2, FTSJD2, hMTr1, interferon-stimulated ge
between 1 and 50
2 - 8°C
Detection of human and mouse KIAA0082 by western blot. Samples: Whole cell lysate (50 µg) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-KIAA0082 antibody A300-303A (lot A300-303A-2) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Detection of human KIAA0082 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-KIAA0082 antibody A300-303A (lot A300-303A-2) used for IP at 6 µg per reaction. KIAA0082 was also immunoprecipitated by a previous lot of this antibody (lot A300-304A) and rabbit anti-KIAA0082 antibody A300-305A. For blotting immunoprecipitated KIAA0082, A300-303A was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.