Antibody was affinity purified using an epitope specific to WAPL immobilized on solid support. The epitope recognized by A300-268A maps to a region between residues 1125 and 1175 of human wings apart like/KIAA0261 using the numbering given in TrEMBL entry Q7Z5K2 (GeneID 23063). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
FOE, friend of EBNA2 (Epstein-Barr virus nuclear protein 2), friend of EBNA2 protein, KIAA0261, WAPAL, WAPL cohesin release factor, wings apart-like protein homolog
between 1125 and 1175
2 - 8°C
Detection of human WAPL by western blot. Samples: Whole cell lysate (50 µg) from HeLa, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-WAPL antibody A300-268A (lot A300-268A-4) used for WB at 0.04 µg/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.