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Rabbit anti-Phospho RPA32 (S4/S8) Antibody, Affinity Purified

Catalogue number:
A300-245A
Size:
100 µg
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Immunogen was a dually phosphorylated synthetic peptide, which represented a portion of human replication protein A2, 32 kDa surrounding phosphorylated serines that corresponded to positions 4 and 8 using the numbering given in entry NP_002937.1 (GeneID 6118). Antibody was affinity purified using the peptide immobilized on solid support. Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.

  • Applications: WB, IP, IHC, ICC-IF
Product Type:

Antibodies

Alternative Names:

REPA2, replication factor A protein 2, replication protein A 32 kDa subunit, replication protein A 34 kDa subunit, RF-A protein 2, RP-A p32, RP-A p34, RPA32

Species Reactivity:

Human, Mouse

Immunogen:

surrounding serine 4/8

Storage Temp:

2 - 8°C

Antibody Host:

Rabbit

Antibody Isotype:

Whole IgG

Antibody Type:

Polyclonal

UNSPSC Code:

12352203

Detection of human Phospho RPA32 (S4/S8)

Detection of human Phospho RPA32 (S4/S8) by western blot. Samples: Whole cell lysate (50 µg) from HeLa cells treated with 100 µM etoposide for 4 hours (+) or mock treated (-) cells. Antibodies: Affinity purified rabbit anti-Phospho RPA32 (S4/S8) antibody A300-245A (lot A300-245A-6) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds. Lower panel shows western blot for total RPA32 using affinity purified rabbit anti-RPA32 antibody A300-244A at 0.1 µg/ml with an exposure time of 30 seconds.

Detection of human Phospho RPA32 (S4/S8)

Detection of human Phospho RPA32 (S4/S8) by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells treated with 100 µM etoposide for 4 hours (+) or mock treated (-) cells. Antibodies: RPA32 (S4/S8) was immunoprecipitated using affinity purified rabbit anti-Phospho RPA32 (S4/S8) antibody A300-245A (lot A300-245A-6). For blotting immunoprecipitated Phospho RPA32 (S4/S8), affinity purified rabbit anti-RPA32 antibody A300-244A was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Detection of human Phospho RPA32 (S4/S8)

Detection of human Phospho RPA32 (S4/S8) by immunohistochemistry. Sample: FFPE section of human lung cancer. Antibody: Affinity purified rabbit anti- Phospho RPA32 (S4/S8) (Cat. No. A300-245A Lot6) used at a dilution of 1:1,000 (1µg/ml). Detection: DAB

Detection of mouse Phospho RPA32 (S4/S8)

Detection of mouse Phospho RPA32 (S4/S8) by immunohistochemistry. Sample: FFPE section of mouse teratoma. Antibody: Affinity purified rabbit anti- Phospho RPA32 (S4/S8) (Cat. No. A300-245A Lot6) used at a dilution of 1:1,000 (1µg/ml). Detection: DAB

Detection of human Phospho RPA32 (S4/S8)

Detection of human Phospho RPA32 (S4/S8) by immunocytochemistry. Samples: Formaldehyde-fixed asynchronous HeLa cells. Untreated cells (left) and etoposide-treated cells (right). Antibody: Affinity purified rabbit anti- Phospho RPA32 (S4/S8) (Cat. No. A300-245A Lot 4) used at a dilution of 1:1,000 (1µg/ml). Detection: Red-fluorescent goat anti-rabbit IgG-heavy and light chain cross-adsorbed Antibody DyLight® 594 Conjugated used at a dilution of 1:100. Counterstain: Alexa Fluor® 488 phalloidin (green).