Tel: +44 (0)1223 316 855
life science research products, biological research products, biotechnology
close ...


Supplier:
Product types:
Bromodomain-Containing (BRD) Antibodies
Over 100,000 Antibodies Performance Guaranteed

Assemble DNA Constructs Simply & Efficiently Using The Gibson Assembly® Method

Rabbit anti-Phospho RPA32 (S4/S8) Antibody, Affinity Purified - A300-245A - 100 µg - Bethyl Laboratories, Inc. - antibodies

Rabbit anti-Phospho RPA32 (S4/S8) Antibody, Affinity Purified
print page
Size: 100 µg
£322.00
GBP | Euro | USD
Add to basket
Catalogue number:
A300-245A
Name:
Rabbit anti-Phospho RPA32 (S4/S8) Antibody, Affinity Purified
Supplier:
Availability:

Immunogen was a dually phosphorylated synthetic peptide, which represented a portion of human replication protein A2, 32 kDa surrounding phosphorylated serines that corresponded to positions 4 and 8 using the numbering given in entry NP_002937.1 (GeneID 6118).Antibody was affinity purified using the peptide immobilized on solid support. Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.

Applications:

WB, IP, IHC, ICC-IF

Product Type:
Antibodies
Alternative Names:
REPA2, replication factor A protein 2, replication protein A 32 kDa subunit, replication protein A 34 kDa subunit, RF-A protein 2, RP-A p32, RP-A p34, RPA32
Species Reactivity:
WB, IP, IHC, ICC-IF
Immunogen:
Surrounding serine 4/8
Storage Temp:
2 - 8°C
Antibody Host:
WB, IP, IHC, ICC-IF
Antibody Isotype:
Whole IgG
Antibody Type:
Polyclonal
UNSPSC Code:
12352203
thumbnail

Detection of human Phospho RPA32 (S4/S8) by western blot. Samples: Whole cell lysate (50 µg) from HeLa cells treated with 100 µM etoposide for 4 hours (+) or mock treated (-) cells. Antibodies: Affinity purified rabbit anti-Phospho RPA32 (S4/S8) antibody A300-245A (lot A300-245A-6) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds. Lower panel shows western blot for total RPA32 using affinity purified rabbit anti-RPA32 antibody A300-244A at 0.1 µg/ml with an exposure time of 30 seconds.

thumbnail

Detection of human Phospho RPA32 (S4/S8) by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells treated with 100 µM etoposide for 4 hours (+) or mock treated (-) cells. Antibodies: RPA32 (S4/S8) was immunoprecipitated using affinity purified rabbit anti-Phospho RPA32 (S4/S8) antibody A300-245A (lot A300-245A-6). For blotting immunoprecipitated Phospho RPA32 (S4/S8), affinity purified rabbit anti-RPA32 antibody A300-244A was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

thumbnail

Detection of human Phospho RPA32 (S4/S8) by immunohistochemistry. Sample: FFPE section of human lung cancer. Antibody: Affinity purified rabbit anti- Phospho RPA32 (S4/S8) (Cat. No. A300-245A Lot6) used at a dilution of 1:1,000 (1µg/ml). Detection: DAB

thumbnail

Detection of mouse Phospho RPA32 (S4/S8) by immunohistochemistry. Sample: FFPE section of mouse teratoma. Antibody: Affinity purified rabbit anti- Phospho RPA32 (S4/S8) (Cat. No. A300-245A Lot6) used at a dilution of 1:1,000 (1µg/ml). Detection: DAB

thumbnail

Detection of human Phospho RPA32 (S4/S8) by immunocytochemistry. Samples: Formaldehyde-fixed asynchronous HeLa cells. Untreated cells (left) and etoposide-treated cells (right). Antibody: Affinity purified rabbit anti- Phospho RPA32 (S4/S8) (Cat. No. A300-245A Lot 4) used at a dilution of 1:1,000 (1µg/ml). Detection: Red-fluorescent goat anti-rabbit IgG-heavy and light chain cross-adsorbed Antibody DyLight® 594 Conjugated used at a dilution of 1:100. Counterstain: Alexa Fluor® 488 phalloidin (green).

Contact Our Customer Service Team