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  3. A300-245A
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Rabbit anti-Phospho RPA32 (S4/S8) Antibody, Affinity Purified

Catalogue number:
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A300-245A
Supplier:
Bethyl Laboratories, Inc.
Size:
100 µl (1000 µg/ml) _$$_
Product is available in:
  • UK
  • Ireland
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£464.00
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Estimated delivery Fri 3 May
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Applications:

WB, IP, IHC, ICC-IF

Antibody Host:

Rabbit

Target:

RPA32, Phospho (S4/S8)

Species Reactivity:

Human; Mouse

Antibody Type:

Polyclonal

Immunogen:

surrounding serine 4/8

Alternative Names:

replication factor A protein 2; RPA32; RP-A p34; RP-A p32; RF-A protein 2; replication protein A 32 kDa subunit; REPA2; replication protein A 34 kDa subunit

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Product Description:

Rabbit anti-Phospho RPA32 (S4/S8) Antibody, Affinity Purified - 100 µl (1000 µg/ml)

Storage Temperature:

2 - 8°C

Detection of human Phospho RPA32 (S4/S8)
Detection of human Phospho RPA32 (S4/S8) by western blot. Samples: Whole cell lysate (50 µg) from HeLa cells treated with 100 µM etoposide for 4 hours (+) or mock treated (-) cells. Antibodies: Affinity purified rabbit anti-Phospho RPA32 (S4/S8) antibody A300-245A (lot A300-245A-6) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds. Lower panel shows western blot for total RPA32 using affinity purified rabbit anti-RPA32 antibody A300-244A at 0.1 µg/ml with an exposure time of 30 seconds.
Detection of human Phospho RPA32 (S4/S8)
Detection of human Phospho RPA32 (S4/S8) by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells treated with 100 µM etoposide for 4 hours (+) or mock treated (-) cells. Antibodies: RPA32 (S4/S8) was immunoprecipitated using affinity purified rabbit anti-Phospho RPA32 (S4/S8) antibody A300-245A (lot A300-245A-6). For blotting immunoprecipitated Phospho RPA32 (S4/S8), affinity purified rabbit anti-RPA32 antibody A300-244A was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Detection of human Phospho RPA32 (S4/S8)
Detection of human Phospho RPA32 (S4/S8) by immunohistochemistry. Sample: FFPE section of human lung cancer. Antibody: Affinity purified rabbit anti- Phospho RPA32 (S4/S8) (Cat. No. A300-245A Lot6) used at a dilution of 1:1,000 (1µg/ml). Detection: DAB
Detection of mouse Phospho RPA32 (S4/S8)
Detection of mouse Phospho RPA32 (S4/S8) by immunohistochemistry. Sample: FFPE section of mouse teratoma. Antibody: Affinity purified rabbit anti- Phospho RPA32 (S4/S8) (Cat. No. A300-245A Lot6) used at a dilution of 1:1,000 (1µg/ml). Detection: DAB
Detection of human Phospho RPA32 (S4/S8)
Detection of human Phospho RPA32 (S4/S8) by immunocytochemistry. Samples: Formaldehyde-fixed asynchronous HeLa cells. Untreated cells (left) and etoposide-treated cells (right). Antibody: Affinity purified rabbit anti- Phospho RPA32 (S4/S8) (Cat. No. A300-245A Lot 4) used at a dilution of 1:1,000 (1µg/ml). Detection: Red-fluorescent goat anti-rabbit IgG-heavy and light chain cross-adsorbed Antibody DyLight® 594 Conjugated used at a dilution of 1:100. Counterstain: Alexa Fluor® 488 phalloidin (green).
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