Antibody was affinity purified using an epitope specific to ZHX1 immobilized on solid support. The epitope recognized by A300-243A maps to a region between residues 1 and 50 of human zinc fingers and homeoboxes 1 using the numbering given in TrEMBL entry Q9UKY1 (GeneID 11244). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
zinc finger and homeodomain protein 1, zinc fingers and homeobox 1, zinc fingers and homeoboxes protein 1
between 1 and 50
2 - 8°C
Detection of human and mouse ZHX1 by western blot. Samples: Whole cell lysate (15 µg) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-ZHX1 antibody A300-243A (lot A300-243A-2) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Detection of human ZHX1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-ZHX1 antibody A300-243A (lot A300-243A-2) used for IP at 3 µg per reaction. ZHX1 was also immunoprecipitated by rabbit anti-ZHX1 antibody BL1012. For blotting immunoprecipitated ZHX1, A300-243A was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.