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Rabbit anti-Tyro3 Antibody, Affinity Purified

Catalogue number:
Size:
100 µg _$$_
Product is available in:
  • UK
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Applications:

WB, IP

Antibody Host:

Rabbit

Species Reactivity:

Human

Antibody Isotype:

Whole IgG

Antibody Type:

Polyclonal

Immunogen:

Between 850 and C-term

Alternative Names:

BYK, Dtk, Etk-2, Rek, RSE, Sky, Tif, tyrosine-protein kinase byk, tyrosine-protein kinase DTK, tyrosine-protein kinase receptor TYRO3, tyrosine-protein kinase RSE, tyrosine-protein kinase SKY, tyrosine-protein kinase TIF

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Product Description:

Antibody was affinity purified using an epitope specific to Tyro3 immobilized on solid support.The epitope recognized by A300-242A maps to a region between residues 850 and the C-terminus (residue 890) of human Tyro3 Tyrosine Kinase using the numbering given in Swiss-Prot entry Q06418 (GeneID 7301).Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.

Storage Temperature:

2 - 8°C

Detection of human Tyro3 by western blot
Detection of human Tyro3 by western blot. Samples: Whole cell lysate (50 µg) from U937 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Tyro3 antibody A300-242A (lot A300-242A-1) used for WB at 0.2 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Detection of human Tyro3 by western blot
Detection of human Tyro3 by western blot and immunoprecipitation. Samples: Whole cell lysate (50 µg for A; 500 µg for B) from U937 cells. Antibody: Affinity purified rabbit anti-Tyro3 antibody BL1154 (Cat. No. A300-242A) used at the indicated concentrations for WB (A and B) and at 10 µg/mg of lysate for IP (B). Another rabbit polyclonal antibody was used in IP as a negative control (Ctrl IP). Detection: Chemiluminescence with exposure times of 5 minutes (A) and 30 minutes (B).
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