Antibody was affinity purified using an epitope specific to BHC110/LSD1 immobilized on solid support. The epitope recognized by A300-216A maps to a region between residues 825 and the C-terminus (residue 886) of human FAD-binding protein BRAF35-HDAC complex 110 kDa (lysine-specific histone demethylase 1) using the numbering given in TrEMBL entry O60341 (GeneID 23028). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
amine oxidase (flavin containing) domain 2, AOF2, BHC110, BRAF35-HDAC complex protein BHC110, CPRF, FAD-binding protein BRAF35-HDAC complex, 110 kDa subunit, flavin-containing amine oxidase domain-containing protein 2, KDM1, LSD1, lysine (K)-specific deme
between 825 and C-term
2 - 8°C
Detection of human BHC110/LSD1 by western blot. Samples: Whole cell lysate (50 µg) from HeLa and HEK293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-BHC110/LSD1 antibody A300-216A (lot A300-216A-1) used for WB at 0.06 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.