Antibody was affinity purified using an epitope specific to FKBP-135 immobilized on solid support. The epitope recognized by A300-211A maps to a region between residues 1175 and the C-terminus (residue 1234) of human FK506-binding protein 135 using the numbering given in TrEMBL entry Q9Y4D0 (GeneID 23307). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
133 kDa FK506-binding protein, 133 kDa FKBP, FK506 binding protein 15, 133kDa, FK506-binding protein 133kDa, FK506-binding protein 15, FKBP133, FKBP-133, FKBP-15, KIAA0674, PPP1R76, protein phosphatase 1, regulatory subunit 76, WAFL, WASP- and FKBP-like p
between 1175 and C-term
2 - 8°C
Detection of human FKBP-135 by western blot and immunoprecipitation. Samples: Nuclear extract (NE; 1 mg for IP; 25 µg for WB) from HeLa cells. Antibodies: Affinity purified rabbit anti-FKBP-135 antibodies BL1008 (Cat. No. A300-209A), BL1009 (Cat. No. A300-210A) and BL1010 (Cat. No. A300-211A) used at 2 µg/mg extract for IP. FKBP-135 in NE and immunoprecipitated FKBP-135 were detected using BL1008 (Cat. No. A300-209A) at 0.2 µg/ml. Detection: Chemiluminescence with an exposure time of 10 minutes.