Antibody was affinity purified using an epitope specific to FKBP-135 immobilized on solid support. The epitope recognized by A300-209A maps to a region between residues 50 and 100 of human FK506-binding protein 135 using the numbering given in TrEMBL entry Q9Y4D0 (GeneID 23307) Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
133 kDa FK506-binding protein, 133 kDa FKBP, FK506 binding protein 15, 133kDa, FK506-binding protein 133kDa, FK506-binding protein 15, FKBP133, FKBP-133, FKBP-15, KIAA0674, PPP1R76, protein phosphatase 1, regulatory subunit 76, WAFL, WASP- and FKBP-like p
between 50 and 100
2 - 8°C
Detection of human and mouse FKPB-135 by western blot. Samples: Whole cell lysate (50 µg) from HeLa, HEK293T, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-FKPB-135 antibody A300-209A (lot A300-209A-2) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Detection of human FKPB-135 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-FKPB-135 antibody A300-209A (lot A300-209A-2) used for IP at 6 µg per reaction. FKPB-135 was also immunoprecipitated by a previous lot of this antibody (A300-209A-1) and rabbit anti-FKPB-135 antibody A300-210A. For blotting immunoprecipitated FKPB-135, A300-209A was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.