Antibody was affinity purified using a phosphorylated epitope corresponding to phosphorylation at Serine 483 and Serine 484 of WASP immobilized on solid support. The epitope recognized by A300-205A is phosphorylated Serine 483, phosphorylated Serine 484 and surrounding residues of human Wiskott-Aldrich syndrome using the numbering given in entry NP_000368.1 (GeneID 7454). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
eczema-thrombocytopenia, IMD2, SCNX, THC, THC1, thrombocytopenia 1 (X-linked), WASP, WASPA, wiskott-Aldrich syndrome protein
surrounding serine 483/484
2 - 8°C
Detection of human Phospho WASP (S483/S484) by western blot. Samples: Whole cell lysate (50 µg) from Jurkat and HEK293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Phospho WASP (S483/S484) antibody A300-205A (lot A300-205A-2) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.