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Rabbit anti-TRIP4/ASC-1 Antibody, Affinity Purified

Catalogue number:
Size:
100 µl (1000 µg/ml) _$$_
Product is available in:
  • UK
  • Ireland
  • Europe
  • USA
  • Rest of World
£436.00 Estimated delivery Fri 3 May
Shipping is calculated in checkout
Applications:

WB, IP

Antibody Host:

Rabbit

Target:

TRIP4/ASC-1

Species Reactivity:

Human

Antibody Type:

Polyclonal

Immunogen:

between 75 and 125

Alternative Names:

SMABF1; zinc finger, C2HC5-type; ZC2HC5; TRIP-4; thyroid receptor-interacting protein 4; MDCDC; HsT17391; ASC-1; ASC1; activating signal cointegrator 1; TR-interacting protein 4

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Product Description:

Rabbit anti-TRIP4/ASC-1 Antibody, Affinity Purified - 100 µl (1000 µg/ml)

Storage Temperature:

2 - 8°C

Detection of human TRIP4/ASC1 by western
Detection of human TRIP4/ASC1 by western blot. Samples: Whole cell lysate (50 µg) from HeLa and HEK293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-TRIP4/ASC1 antibody A300-203A (lot A300-203A-1) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Detection of human TRIP4/ASC1 by western
Detection of human TRIP4/ASC1 by western blot and immunoprecipitation. Samples: Nuclear extract (5 µg for WB; 1 mg for IP) from HeLa cells. Antibodies: Affinity purified rabbit anti-ASC1 antibody (Cat. No. A300-203A) used at 0.3 µg/ml for WB and 4 µg/mg lysate for IP. immunoprecipitatesd ASC1 was detected using an antibody (BL458) to a distinctly different epitope on ASC1. Detection: A) An alkaline phosphatase conjugated secondary antibody with NBT-BCIP development for 20 minutes. B) An HRP conjugated secondary antibody with chemiluminescent exposure for 4 minute.
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