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Rabbit anti-CKII alpha Antibody, Affinity Purified - A300-198A - 100 µg - Bethyl Laboratories, Inc. - antibodies

Rabbit anti-CKII alpha Antibody, Affinity Purified
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Size: 100 µg
£302.00
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Catalogue number:
A300-198A
Name:
Rabbit anti-CKII alpha Antibody, Affinity Purified
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Availability:

Antibody was affinity purified using an epitope specific to CKII alpha immobilized on solid support. The epitope recognized by A300-198A maps to a region between residue 350 and the C-terminus (residue 391) of human Casein Kinase 2, alpha 1 using the numbering given in entry NP_001886.1 (GeneID 1457).Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.

Applications:

WB, IP, IHC

Product Type:
Antibodies
Alternative Names:
casein kinase 2, alpha 1 polypeptide, casein kinase II alpha 1 polypeptide pseudogene, casein kinase II alpha 1 subunit, casein kinase II subunit alpha, CK II alpha, CK2 catalytic subunit alpha, CK2A1, Cka1, Cka2, CKII, OCNDS, protein kinase CK2
Species Reactivity:
Human, Mouse
Immunogen:
Between 350 and C-term
Storage Temp:
2 - 8°C
Antibody Host:
Rabbit
Antibody Isotype:
Whole IgG
Antibody Type:
Polyclonal
UNSPSC Code:
12352203
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Detection of human and mouse CKII alpha by western blot. Samples: Whole cell lysate (50 µg) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells. Antibodies: Affinity purified rabbit anti-CKII alpha antibody A300-198A (lot A300-198A-2) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.

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Detection of human CKII alpha by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-CKII alpha antibody A300-198A (lot A300-198A-2) used for IP at 6 µg/mg lysate. CKII alpha was also immunoprecipitated by a previous lot (lot A300-198A-1) of this antibody. For blotting immunoprecipitated CKII alpha, A300-198A was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.

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Detection of human CKII Alpha by immunohistochemistry. Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-CKII Alpha (Cat. No. A300-198A Lot2) used at a dilution of 1:5,000 (0.2µg/ml). Detection: DAB

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