Antibody was affinity purified using an epitope specific to CKII alpha immobilized on solid support. The epitope recognized by A300-198A maps to a region between residue 350 and the C-terminus (residue 391) of human Casein Kinase 2, alpha 1 using the numbering given in entry NP_001886.1 (GeneID 1457). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
casein kinase 2, alpha 1 polypeptide, casein kinase II alpha 1 polypeptide pseudogene, casein kinase II alpha 1 subunit, casein kinase II subunit alpha, CK II alpha, CK2 catalytic subunit alpha, CK2A1, Cka1, Cka2, CKII, OCNDS, protein kinase CK2
between 350 and C-term
2 - 8°C
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Detection of human and mouse CKII alpha by western blot. Samples: Whole cell lysate (50 µg) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells. Antibodies: Affinity purified rabbit anti-CKII alpha antibody A300-198A (lot A300-198A-2) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.
Detection of human CKII alpha by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-CKII alpha antibody A300-198A (lot A300-198A-2) used for IP at 6 µg/mg lysate. CKII alpha was also immunoprecipitated by a previous lot (lot A300-198A-1) of this antibody. For blotting immunoprecipitated CKII alpha, A300-198A was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.