Antibody was affinity purified using an epitope specific to CKII alpha immobilized on solid support. The epitope recognized by A300-196A maps to a region between residues 1 and 50 of human Casein Kinase 2, alpha 1 using the numbering given in entry NP_001886.1 (GeneID 1457). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
casein kinase 2, alpha 1 polypeptide, casein kinase II alpha 1 polypeptide pseudogene, casein kinase II alpha 1 subunit, casein kinase II subunit alpha, CK II alpha, CK2 catalytic subunit alpha, CK2A1, Cka1, Cka2, CKII, OCNDS, protein kinase CK2
between 1 and 50
2 - 8°C
Detection of human CKII alpha by western blot. Samples: Whole cell lysate (50 µg) from HeLa and HEK293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-CKII alpha antibody A300-196A (lot A300-196A-1) used for WB at 0.2 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Detection of human CKII Alpha by western blot. Sample: RIPA extract (30 µg) from HeLa cells treated with CKII alpha siRNA or vimentin siRNA (v). Antibody: Affinity purified rabbit anti-CKII alpha (Cat. No. A300-196A) used at 0.33 µg/ml. Detection: Chemiluminescence with a 1 minute exposure.