Antibody was affinity purified using an epitope specific to MCM6 immobilized on solid support. The epitope recognized by A300-194A maps to a region between residue 775 and the C-terminus (residue 821) of human Minichromosome Maintenance 6 using the numbering given in entry NP_005906.2 (GeneID 4175). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
DNA replication licensing factor MCM6, MCG40308, MCM6 minichromosome maintenance deficient 6 (MIS5 homolog, S. pombe), minichromosome maintenance deficient (mis5, S. pombe) 6, Mis5, P105MCM
between 775 and C-term
2 - 8°C
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide