Antibody was affinity purified using an epitope specific to MCM2 immobilized on solid support. The epitope recognized by A300-191A maps to a region between residues 1 and 50 of human human minichromosomal maintenance deficient 2 using the numbering given in entry NP_0045117.2 (GeneID 4171). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
BM28, CCNL1, cdc19, CDCL1, cell devision cycle-like 1, cyclin-like 1, D3S3194, DFNA70, DNA replication licensing factor MCM2, minichromosome maintenance deficient 2 (mitotin), minichromosome maintenance protein 2 homolog, MITOTIN, nuclear protein BM28
between 1 and 50
2 - 8°C
Detection of human and mouse MCM2 by western blot. Samples: Whole cell lysate (50 µg) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-MCM2 antibody A300-191A (lot A300-191A-2) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.
Detection of human MCM2 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-MCM2 antibody A300-191A (lot A300-191A-2) used for IP at 3 µg per reaction. MCM2 was also immunoprecipitated by rabbit anti-Phospho MCM2 (S108) antibody A300-094A. For blotting immunoprecipitated MCM2, A300-191A was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.
Detection of human MCM2 by immunohistochemistry. Sample: FFPE section of human small cell lung cancer. Antibody: Affinity purified rabbit anti-MCM2 (Cat. No. A300-191A Lot2) used at a dilution of 1:1,000 (1µg/ml). Detection: DAB