Antibody was affinity purified using an epitope specific to Rad50 immobilized on solid support. The epitope recognized by A300-185A maps to a region between residues 700 and 750 of human Rad50 using the numbering given in entry NP_005723.2 (GeneID 10111). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
DNA repair protein RAD50, hRad50, NBSLD, RAD50 homolog, double strand break repair protein, RAD502
between 700 and 750
2 - 8°C
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Detection of human Rad50 by western blot. Samples: Whole cell lysate (50 µg) from HeLa and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Rad50 antibody A300-185A (lot A300-185A-1) used for WB at 0.2 µg/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.