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Rabbit anti-Rad50 Antibody, Affinity Purified

Catalogue number:
100 µg
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Antibody was affinity purified using an epitope specific to Rad50 immobilized on solid support. The epitope recognized by A300-185A maps to a region between residues 700 and 750 of human Rad50 using the numbering given in entry NP_005723.2 (GeneID 10111). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.

  • Applications: WB, IHC
Product Type:


Alternative Names:

DNA repair protein RAD50, hRad50, NBSLD, RAD50 homolog, double strand break repair protein, RAD502

Species Reactivity:



between 700 and 750

Storage Temp:

2 - 8°C

Antibody Host:


Antibody Isotype:

Whole IgG

Antibody Type:




Detection of human Rad50 by western blot

Detection of human Rad50 by western blot. Samples: Whole cell lysate (50 µg) from HeLa and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Rad50 antibody A300-185A (lot A300-185A-1) used for WB at 0.2 µg/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.

Detection of human Rad50 by immunohistoc

Detection of human Rad50 by immunohistochemistry. Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti- Rad50 (Cat. No. A300-185A Lot1) used at a dilution of 1:1,000 (1µg/ml). Detection: DAB