Antibody was affinity purified using an epitope specific to MTA3 immobilized on solid support. The epitope recognized by A300-160A maps to a region between residues 400 and 450 of human metastasis associated 3 using the numbering given in entry Swiss-Prot entry Q9BTC8 (GeneID 57504). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
metastasis associated gene family, member 3, metastasis-associated protein MTA3
between 400 and 450
2 - 8°C
Detection of human MTA3 by western blot and immunoprecipitation. Samples: Nuclear extract (25 µg for WB; 0.5 mg for IP) from MCF-7 cells. Antibody: Affinity purified rabbit anti-MTA3 antibody (Cat. No. A300-160A) used at 1 µg/ml for WB and 5 µg/0.5 mg lysate for IP. Detection: Chemiluminescence with exposure times of less than 1 minute.
Detection of human MTA3 by immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti- MTA3 (Cat. No. A300-160A Lot1) used at a dilution of 1:1,000 (1µg/ml). Detection: DAB