Antibody was affinity purified using an epitope specific to SA2 immobilized on solid support. The epitope recognized by A300-158A maps to a region between residues 1100 and 1150 of human Stromal Antigen 2 using the numbering given in entry NP_006594.3 (GeneID 10735). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
bA517O1.1, cohesin subunit SA-2, SA2, SA-2, SCC3 homolog 2, SCC3B, Stromal antigen 2
between 1100 and 1150
2 - 8°C
Detection of human and mouse SA2 by western blot. Samples: Whole cell lysate (50 µg) from HeLa, HEK293T, Jurkat, and mouse TCMK-1 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-SA2 antibody A300-158A (lot A300-158A-2) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Localization of human SA2. Sample: HeLa cells that were extracted for 5 min. at 4C in 0.5% Triton in CSK buffer. Antibody: Affinity purified goat anti-SA2 (BL146G; Cat. No. A300-158A) used at 1 µg/ml.