Antibody was affinity purified using an epitope specific to RFC4 immobilized on solid support. The epitope recognized by A300-145A maps to a region between residues 300 and 350 of human Replication Factor C subunit 4 using the numbering given in entry NP_002907.1 (GeneID 5984). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
A1, A1 37 kDa subunit, activator 1 37 kDa subunit, activator 1 subunit 4, replication factor C (activator 1) 4, 37kDa, replication factor C 37 kDa subunit, replication factor C subunit 4, RFC 37 kDa subunit, RF-C 37 kDa subunit, RFC37
between 300 and 350
2 - 8°C
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Detection of human and mouse RFC4 by western blot. Samples: Whole cell lysate (50 µg) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibodies: Affinity purified goat anti-RFC4 antibody A300-145A (lot A300-145A-2) used for WB at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.