Goat anti-RFC1 Antibody, Affinity Purified - A300-141A - 100 µg - Bethyl Laboratories, Inc. - antibodies
Antibody was affinity purified using an epitope specific to RFC1 immobilized on solid support. The epitope recognized by A300-141A maps to a region between residue 1100 and the C-terminus (residue 1148) of human Replication Factor C subunit 1 using the numbering given in entry NP_002904.3 (GeneID 5981). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
Applications:
WB, IP
Detection of human RFC1 by western blot. Samples: Whole cell lysate (50, 15 µg) from HeLa and HEK293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-RFC1 antibody A300-141A (lot A300-141A-2) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Detection of human RFC1 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-RFC1 antibody A300-141A (lot A300-141A-2) used for IP at 6 µg per reaction. RFC1 was also immunoprecipitated by rabbit anti-RFC1 antibody A300-320A. For blotting immunoprecipitated RFC1, A300-141A was used at 0.4 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.
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