Antibody was affinity purified using an epitope specific to RFC1 immobilized on solid support. The epitope recognized by A300-141A maps to a region between residue 1100 and the C-terminus (residue 1148) of human Replication Factor C subunit 1 using the numbering given in entry NP_002904.3 (GeneID 5981). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
A1, A1 140 kDa subunit, activator 1 140 kDa subunit, activator 1 large subunit, activator 1 subunit 1, DNA-binding protein PO-GA, MHC binding factor, beta, MHCBFB, PO-GA, RECC1, replication factor C (activator 1) 1, 145kDa, replication factor C 140 kDa su
between 1100 and C-term
2 - 8°C
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Detection of human RFC1 by western blot. Samples: Whole cell lysate (50, 15 µg) from HeLa and HEK293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-RFC1 antibody A300-141A (lot A300-141A-2) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Detection of human RFC1 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-RFC1 antibody A300-141A (lot A300-141A-2) used for IP at 6 µg per reaction. RFC1 was also immunoprecipitated by rabbit anti-RFC1 antibody A300-320A. For blotting immunoprecipitated RFC1, A300-141A was used at 0.4 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.