Antibody was affinity purified using the antigen immobilized on solid support. The epitope recognized by A300-134A maps to a region between residue 750 and the C-terminus (residue 785) using the numbering given in entry XP_028606.4 (GeneID 6667). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
specificity protein 1, transcription factor Sp1
between 750 and C-term
2 - 8°C
Detection of human and mouse SP1 by western blot. Samples: Whole cell lysate (50 µg) from HeLa, HEK293T, and mouse Renca cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-SP1 antibody A300-134A (lot A300-134A-5) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.
Detection of human SP1 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-SP1 antibody A300-134A (lot A300-134A-5) used for IP at 6 µg per reaction. SP1 was also immunoprecipitated by a previous lot of this antibody (A300-134A-4) and rabbit anti-SP1 antibody A303-944A. For blotting immunoprecipitated SP1, A300-134A was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.
Localization of Sp1 Binding Sites by ChIP-sequencing. Chromatin from acute myeloid leukemia cell line was immunoprecipitated with anti-Sp1 antibody A300-134A and analyzed by DNA sequencing. The figure illustrates the peak distribution of Sp1 binding within a 250 Kb region of chromosome 6 as detected using anti-Sp1 A300-134A. ChIP-seq validation performed by Active Motif, Carlsbad, CA.