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  2. Bethyl Laboratories, Inc.
  3. Rabbit anti-Sp1 Antibody, Affinity Purified
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Rabbit anti-Sp1 Antibody, Affinity Purified

Catalogue number:
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A300-133A
Supplier:
Bethyl Laboratories, Inc.
Size:
100 µl (1000 µg/ml) _$$_
Product is available in:
  • UK
  • Ireland
  • Europe
  • USA
  • Rest of World
£436.00
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Estimated delivery Wed 15 May
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Applications:

WB, IP, IHC

Antibody Host:

Rabbit

Target:

Sp1

Species Reactivity:

Human; Mouse

Antibody Type:

Polyclonal

Immunogen:

between 700 and 740

Alternative Names:

specificity protein 1; transcription factor Sp1

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Product Description:

Rabbit anti-Sp1 Antibody, Affinity Purified - 100 µl (1000 µg/ml)

Storage Temperature:

2 - 8°C

Detection of human SP1 by western blot.
Detection of human SP1 by western blot. Samples: Whole cell lysate (50 µg) from HeLa and HEK293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-SP1 antibody A300-133A (lot A300-133A-1) used for WB at 0.04 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Detection of human and mouse SP1 by immu
Detection of human and mouse SP1 by immunohistochemistry. Sample: FFPE sections of human breast carcinoma (left) and mouse squamous cell carcinoma (right). Antibody: Affinity purified rabbit anti- Sp1 (Cat. No. A300-133A Lot1) used at a dilution of 1:1,000 (1 µg/ml). Detection: DAB
Detection of human SP1 by western blot a
Detection of human SP1 by western blot and immunoprecipitation. Samples: A. Whole cell lysate (50 µg for E; 10 µg for T) from HEK HEK293T cells that were mock transfected (E) or transfected with an Sp1 expression construct (T). B. Whole cell lysate (~700 µg) from HEK 293T cells. Antibody: A. Affinity purified rabbit anti-Sp1 antibody A300-133A used at the indicated concentrations. B. SP1 was immunoprecipitated using affinity purified rabbit anti-SP1 antibodies BL937, A300-133A, or A300-134A using each antibody at 3.75 µg/mg lysate. Subsequently, A300-133A was used at 0.04 µg/ml for WB. Detection: Chemiluminescence with an exposure time of 10 seconds (A) or 1 min (B).
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