Antibody was affinity purified using the antigen immobilized on solid support. The epitope recognized by A300-133A maps to a region between residues 700 and 740 using the numbering given in entry XP_028606.4 (GeneID 6667). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
specificity protein 1, transcription factor Sp1
between 700 and 740
2 - 8°C
Detection of human SP1 by western blot. Samples: Whole cell lysate (50 µg) from HeLa and HEK293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-SP1 antibody A300-133A (lot A300-133A-1) used for WB at 0.04 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Detection of human and mouse SP1 by immunohistochemistry. Sample: FFPE sections of human breast carcinoma (left) and mouse squamous cell carcinoma (right). Antibody: Affinity purified rabbit anti- Sp1 (Cat. No. A300-133A Lot1) used at a dilution of 1:1,000 (1 µg/ml). Detection: DAB
Detection of human SP1 by western blot and immunoprecipitation. Samples: A. Whole cell lysate (50 µg for E; 10 µg for T) from HEK HEK293T cells that were mock transfected (E) or transfected with an Sp1 expression construct (T). B. Whole cell lysate (~700 µg) from HEK 293T cells. Antibody: A. Affinity purified rabbit anti-Sp1 antibody A300-133A used at the indicated concentrations. B. SP1 was immunoprecipitated using affinity purified rabbit anti-SP1 antibodies BL937, A300-133A, or A300-134A using each antibody at 3.75 µg/mg lysate. Subsequently, A300-133A was used at 0.04 µg/ml for WB. Detection: Chemiluminescence with an exposure time of 10 seconds (A) or 1 min (B).