Antibody was affinity purified using an epitope specific to CoREST immobilized on solid support. The epitope recognized by A300-130A maps to a region between residues 425 and the C-terminus (residue 482) of human REST (RE1-silencing transcription factor) corepressor using the numbering given in entry NP_055971.1 (GeneID 23186) Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
COREST, Protein CoREST, RCOR, REST corepressor 1
between 425 and C-term
2 - 8°C
Detection of human CoREST by western blot. Samples: Whole cell lysate (50 µg) from HeLa and HEK293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-CoREST antibody A300-130A (lot A300-130A-1) used for WB at 0.66 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Detection of human CoREST by immunohistochemistry. Sample: FFPE section of human skin carcinoma. Antibody: Affinity purified rabbit anti-CoREST (Cat. No. A300-130A) used at a dilution of 1:5,000 (0.2µg/ml). Detection: DAB