Antibody was affinity purified using an epitope specific to MCM7 immobilized on solid support. The epitope recognized by A300-128A maps to a region between residues 300 and 350 of human Minichromosome Maintenance 7 using the numbering given in entry NP_005907.3 (GeneID 4176). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
CDC47, CDC47 homolog, DNA replication licensing factor MCM7, homolog of S. cerevisiae Cdc47, MCM2, minichromosome maintenance deficient 7, P1.1-MCM3, P1CDC47, P85MCM, PNAS146, PPP1R104, protein phosphatase 1, regulatory subunit 104
between 300 and 350
2 - 8°C
Detection of human and mouse MCM7 by western blot. Samples: Whole cell lysate (50 µg) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibodies: Affinity purified goat anti-MCM7 antibody A300-128A (lot A300-128A-2) used for WB at 0.2 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.