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Rabbit anti-ASH2 Antibody, Affinity Purified - A300-112A - 100 µg - Bethyl Laboratories, Inc. - antibodies

Rabbit anti-ASH2 Antibody, Affinity Purified
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Size: 100 µg
£302.00
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Catalogue number:
A300-112A
Name:
Rabbit anti-ASH2 Antibody, Affinity Purified
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Antibody was affinity purified using an epitope specific to ASH2 immobilized on solid support. The epitope recognized by A300-112A maps to a region between residue 575 and the C-terminus (residue 628) of human Absent, Small, or Homeotic-Like 2 using the numbering given in entry NP_004665.1 (GeneID 9070).Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.

Applications:

WB, IP, IHC

Product Type:
Antibodies
Alternative Names:
ASH2, ash2 (absent, small, or homeotic)-like, ASH2L1, ASH2L2, ASH2-like protein, Bre2, set1/Ash2 histone methyltransferase complex subunit ASH2
Species Reactivity:
Human, Mouse
Immunogen:
Between 225 and 275
Storage Temp:
2 - 8°C
Antibody Host:
Rabbit
Antibody Isotype:
Whole IgG
Antibody Type:
Polyclonal
UNSPSC Code:
12352203
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Detection of human ASH2 by western blot. Samples: Whole cell lysate (15 µg) from HeLa, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-ASH2 antibody A300-112A (lot A300-112A-3) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

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Detection of mouse ASH2 by western blot. Samples: Whole cell lysate (50 µg) from TCMK-1 and NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-ASH2 antibody A300-112A (lot A300-112A-3) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

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Detection of human ASH2 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-ASH2 antibody A300-112A (lot A300-112A-3) used for IP at 3 µg per reaction. ASH2 was also immunoprecipitated by rabbit anti-ASH2 antibody A300-489A. For blotting immunoprecipitated ASH2, A300-112A was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

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Detection of human ASH2 by immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-ASH2 (Cat. No. A300-112A Lot 3) used at a dilution of 1:1,000 (1µg/ml). Detection: DAB

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Detection of mouse ASH2 by immunohistochemistry. Sample: FFPE section of mouse CT26 colon carcinoma. Antibody: Affinity purified rabbit anti-ASH2 (Cat. No. A300-112A Lot 3) used at a dilution of 1:1,000 (1µg/ml). Detection: DAB

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