Antibody was affinity purified using an epitope specific to Menin immobilized on solid support. The epitope recognized by A300-105A maps to a region between residue 575 and the C-terminus (residue 615) of human Multiple Endocrine Neoplasia 1 using the numbering given in entry NP_000235.2 (GeneID 4221). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
MEAI, menin, SCG2
between 575 and C-term
2 - 8°C
Detection of human and mouse Menin by western blot. Samples: Whole cell lysate (50 µg) from HeLa, HEK293T, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Menin antibody A300-105A (lot A300-105A-8) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Detection of human Menin by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-Menin antibody A300-105A (lot A300-105A-8) used for IP at 6 µg per reaction. Menin was also immunoprecipitated goat anti-Menin antibody A300-106A and rabbit anti-Menin antibody A300-115. For blotting immunoprecipitated Menin, A300-105A was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.