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Rabbit anti-EPLIN Antibody, Affinity Purified

Catalogue number:
A300-103A
Size:
100 µg
Product is available in:
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Antibody was affinity purified using an epitope specific to EPLIN immobilized on solid support. The epitope recognized by A300-103A maps to a region between residues 700 and the C-terminus (residue 759) of human epithelial protein lost in neoplasm beta using the numbering given in entry NP_057441.1 (GeneID 51474) Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.

  • Applications: WB, IP
Product Type:

Antibodies

Alternative Names:

1110021C24Rik, Epithelial protein lost in neoplasm, epithelial protein lost in neoplasm beta, EPLIN, LDLCQ8, LIM domain and actin-binding protein 1, SREBP3, sterol regulatory element binding protein 3

Species Reactivity:

Human

Immunogen:

between 700 and C-term

Storage Temp:

2 - 8°C

Antibody Host:

Rabbit

Antibody Isotype:

Whole IgG

Antibody Type:

Polyclonal

UNSPSC Code:

12352203

Detection of human EPLIN by western blot

Detection of human EPLIN by western blot. Samples: Whole cell lysate from MCF-7 (5, 15 and 50 µg) cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-EPLIN antibody A300-103A (lot A300-103A-4) used for WB at 0.4 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Detection of human EPLIN by western blot

Detection of human EPLIN by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from MCF-7 cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-EPLIN antibody A300-103A (lot A300-103A-4) used for IP at 6 µg per reaction. EPLIN was also immunoprecipitated by rabbit anti-EPLIN antibody A300-255A. For blotting immunoprecipitated EPLIN, A300-103A was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.