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  3. A300-094A
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Rabbit anti-Phospho MCM2 (S108) Antibody, Affinity Purified

Catalogue number:
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A300-094A
Supplier:
Bethyl Laboratories, Inc.
Size:
100 µl (1000 µg/ml) _$$_
Product is available in:
  • UK
  • Ireland
  • Europe
  • USA
  • Rest of World
£464.00
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Estimated delivery Fri 3 May
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Applications:

WB, IP, IHC

Antibody Host:

Rabbit

Target:

MCM2, Phospho (S108)

Species Reactivity:

Human; Mouse

Antibody Type:

Polyclonal

Immunogen:

surrounding serine 108

Alternative Names:

D3S3194; MITOTIN; minichromosome maintenance protein 2 homolog; minichromosome maintenance deficient 2 (mitotin); nuclear protein BM28; DFNA70; cell devision cycle-like 1; CDCL1; cdc19; CCNL1; BM28; DNA replication licensing factor MCM2; cyclin-like 1

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Product Description:

Rabbit anti-Phospho MCM2 (S108) Antibody, Affinity Purified - 100 µl (1000 µg/ml)

Storage Temperature:

2 - 8°C

Detection of human and mouse Phospho MCM
Detection of human and mouse Phospho MCM2 (S108) by western blot. Samples: Whole cell lysate (15 or 50 µg) from HEK293T cells or mouse NIH 3T3 (M) cells treated with 100 µM etoposide for 2 hours (+) or mock treated (-) cells. Antibodies: Affinity purified rabbit anti-phospho MCM2 (S108) antibody A300-094A (lot A300-094A-4) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.
Detection of human Phospho MCM2 (S108) b
Detection of human Phospho MCM2 (S108) by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from HEK293T cells either mock treated (-) or treated (+) with etoposide (100 µM, 2h). Antibodies: MCM2 was immunoprecipitated using rabbit anti-MCM2 antibody A300-191A. Following immunoprecipitation the product of the IP reaction was either mock treated (-) or treated with phosphatases (+). Affinity purified rabbit anti-phospho MCM2 (S108) antibody A300-094A (lot A300-094A-4) was used for WB at 1 µg/ml. To examine total MCM2, the blot was stripped and then blotted with rabbit anti-MCM2 antibody A300-191A. Detection: Chemiluminescence with exposure times of 10 seconds (upper and lower panels).
Detection of human and mouse Phospho MCM
Detection of human and mouse Phospho MCM2 (S108) by immunohistochemistry. Sample: FFPE sections of human breast carcinoma (left) and mouse renal cell carcinoma (right). Antibody: Affinity purified rabbit anti- phospho MCM2 (S108) (Cat. No. A300-094A Lot4) used at a dilution of 1:5,000 (0.2µg/ml). Detection: DAB
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