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Rabbit anti-Diaphanous 1 Antibody, Affinity Purified - A300-078A - 100 µg - Bethyl Laboratories, Inc. - antibodies

Rabbit anti-Diaphanous 1 Antibody, Affinity Purified
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Size: 100 µg
£302.00
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Catalogue number:
A300-078A
Name:
Rabbit anti-Diaphanous 1 Antibody, Affinity Purified
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Antibody was affinity purified using an epitope specific to Diaphanous 1 immobilized on solid support. The epitope recognized by A300-078A maps to a region between residues 1200 and the C-terminus (residue 1248) of human Diaphanous Homolog 1 using the numbering given in entry NP_005210.1 (GeneID 1729).Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.

Applications:

WB, IHC

Product Type:
Antibodies
Alternative Names:
DFNA1, DIA1, Diaphanous-related formin-1, DRF1, hDIA1, LFHL1, protein diaphanous homolog 1, SCBMS
Species Reactivity:
Human
Immunogen:
Between 1200 and C-term
Storage Temp:
2 - 8°C
Antibody Host:
Rabbit
Antibody Isotype:
Whole IgG
Antibody Type:
Polyclonal
UNSPSC Code:
12352203
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Detection of human Diaphanous 1 by western blot. Samples: Whole cell lysate (50 µg) from HeLa and HEK293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Diaphanous 1 antibody A300-078A (lot A300-078A-1) used for WB at 0.66 µg/ml. Detection: Chemiluminescence with an exposure time of 1 second.

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Detection of human Diaphanous by immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-Diaphanous (Cat. No. A300-078A) used at a dilution of 1:1,000 ( 1µg/ml). Detection: DAB

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Detection of human Diaphanous 1 by western blot. Samples: Whole cell lysate (50 µg/lane) from untreated or siRNA treated HEK293T cells. Antibody: Affinity purified rabbit anti-Diaphanous 1 antibody A300-078A used at 0.1 µg/ml. Detection: Chemiluminescence with 10 second exposure.

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