Antibody was affinity purified using an epitope specific to Diaphanous 1 immobilized on solid support. The epitope recognized by A300-078A maps to a region between residues 1200 and the C-terminus (residue 1248) of human Diaphanous Homolog 1 using the numbering given in entry NP_005210.1 (GeneID 1729). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
DFNA1, DIA1, Diaphanous-related formin-1, DRF1, hDIA1, LFHL1, protein diaphanous homolog 1, SCBMS
between 1200 and C-term
2 - 8°C
Detection of human Diaphanous 1 by western blot. Samples: Whole cell lysate (50 µg) from HeLa and HEK293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Diaphanous 1 antibody A300-078A (lot A300-078A-1) used for WB at 0.66 µg/ml. Detection: Chemiluminescence with an exposure time of 1 second.