Antibody was affinity purified using an epitope from human PKD2 immobilized on solid support. The epitope recognized by A300-074A maps to a region between residues 825 and the C-terminus (residue 878) using the numbering given in entry NP_057541.2 (GeneID 25865). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
HSPC187, nPKC-D2, PKD2, serine/threonine-protein kinase D2
between 825 and C-term
2 - 8°C
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Detection of human PKD2 by western blot. Samples: Whole cell lysate (50 µg) from HeLa and HEK293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-PKD2 antibody A300-074A (lot A300-074A-1) used for WB at 0.2 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minute
Detection of human PKD2 by western blot and immunoprecipitation. Samples: Whole cell lysate (A. 30 µg/lane; B. 200 µg/IP) from HEK293 cells that were untreated or transfected with expression constructs for PKD1 and PKD3 as controls for reactivity with these homologs. Antibodies: A. Affinity purified rabbit anti-PKD2 antibody A300-074A used at the indicated concentrations for WB. B. Affinity purified rabbit anti-PKD2 antibodies A300-073A and A300-074A used at 2 µg/200 µg lysate for IP. immunoprecipitatesd PKD2 was detected by WB using anti-PKD2 antibody A300-074A at 1 µg/ml. Detection: Chemiluminescence with a 10 second exposure.