Rabbit anti-MDC1 Antibody, Affinity Purified
Rabbit anti-MDC1 Antibody, Affinity Purified - 100 µg
Antibodies
homologue to Drosophila photoreceptor protein calphotin, mediator of DNA damage checkpoint protein 1, NFBD1, nuclear factor with BRCT domains 1
Human
between 475 and 525
2 - 8°C
Rabbit
Whole IgG
Polyclonal
12352203
Detection of human MDC1 by western blot. Samples: Whole cell lysate (15 and 50 µg) from HeLa cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-MDC1 antibody A300-051A (lot A300-051A-3) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Detection of human MDC1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-MDC1 antibody A300-051A (lot A300-051A-3) used for IP at 3 µg per reaction. MDC1 was also immunoprecipitated by rabbit anti-MDC1 antibodies A300-052A and A300-053A. For blotting immunoprecipitated MDC1, A300-051A was used at 0.4 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.