CDLS2, DXS423E, epididymis secretory sperm binding protein, SB1.8, segregation of mitotic chromosomes 1, SMC protein 1A, SMC1, SMC1 (structural maintenance of chromosomes 1, yeast)-like 1, SMC-1A, SMC1alpha, SMC-1-alpha, SMC1L1, SMCB, structural maintenan
surrounding serine 966
2 - 8°C
Detection of Phospho SMC1 (S966) by western blot. Samples: Whole cell lysate (50 µg) from HeLa cells that were treated with NCS (+; 200 ng/ml; 1h) or mock treated (-). Antibodies: Affinity purified rabbit anti-Phospho SMC1 (S966) antibody A300-050A (lot A300-050A-6) used at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes. For detection of total SMC1, rabbit anti-SMC1 antibody A300-055A was used.
Detection of human Phospho SMC1 (S966) by immunocytochemistry. Samples: Neocarzinostatin treated asynchronous HeLa cells. Antibody: Affinity purified rabbit anti-Phospho SMC1 (S966) (Cat. No. A300-050 Lot6) used at a dilution of 1:5,000 (0.2µg/ml). Detection: Red fluorescent Anti-rabbit IgG-DyLight® 594 conjugated used at a dilution of 1:100.