Rabbit anti-Phospho SMC1 (S966) Antibody, Affinity Purified - A300-050A - 100 µg - Bethyl Laboratories, Inc. - antibodies
Antibody was affinity purified using an epitope specific to phosphorylation of SMC1 at serine 966 immobilized on solid support. Immunogen for A300-050A was a phosphorylated synthetic peptide, which represented a portion of human Structural Maintenance of Chromosomes 1 (GeneID 8243) around serine 966 according to the numbering given in entry NP_006297.2.Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
Applications:
WB, IP, IHC, ICC-IF
Detection of Phospho SMC1 (S966) by western blot. Samples: Whole cell lysate (50 µg) from HeLa cells that were treated with NCS (+; 200 ng/ml; 1h) or mock treated (-). Antibodies: Affinity purified rabbit anti-Phospho SMC1 (S966) antibody A300-050A (lot A300-050A-6) used at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes. For detection of total SMC1, rabbit anti-SMC1 antibody A300-055A was used.
Detection of human Phospho SMC1 (S966) by immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti- Phospho SMC1 (S966) (Cat. No. A300-050A Lot6) used at a dilution of 1:1,000 (1µg/ml). Detection: DAB
Detection of human Phospho SMC1 (S966) by immunocytochemistry. Samples: Neocarzinostatin treated asynchronous HeLa cells. Antibody: Affinity purified rabbit anti-Phospho SMC1 (S966) (Cat. No. A300-050 Lot6) used at a dilution of 1:5,000 (0.2µg/ml). Detection: Red fluorescent Anti-rabbit IgG-DyLight® 594 conjugated used at a dilution of 1:100.
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