Antibody was affinity purified using an epitope specific to phosphorylation of SMC1 at serine 966 immobilized on solid support. Immunogen for A300-050A was a phosphorylated synthetic peptide, which represented a portion of human Structural Maintenance of Chromosomes 1 (GeneID 8243) around serine 966 according to the numbering given in entry NP_006297.2. Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
CDLS2, DXS423E, epididymis secretory sperm binding protein, SB1.8, segregation of mitotic chromosomes 1, SMC protein 1A, SMC1, SMC1 (structural maintenance of chromosomes 1, yeast)-like 1, SMC-1A, SMC1alpha, SMC-1-alpha, SMC1L1, SMCB, structural maintenan
surrounding serine 966
2 - 8°C
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Detection of Phospho SMC1 (S966) by western blot. Samples: Whole cell lysate (50 µg) from HeLa cells that were treated with NCS (+; 200 ng/ml; 1h) or mock treated (-). Antibodies: Affinity purified rabbit anti-Phospho SMC1 (S966) antibody A300-050A (lot A300-050A-6) used at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes. For detection of total SMC1, rabbit anti-SMC1 antibody A300-055A was used.
Detection of human Phospho SMC1 (S966) by immunocytochemistry. Samples: Neocarzinostatin treated asynchronous HeLa cells. Antibody: Affinity purified rabbit anti-Phospho SMC1 (S966) (Cat. No. A300-050 Lot6) used at a dilution of 1:5,000 (0.2µg/ml). Detection: Red fluorescent Anti-rabbit IgG-DyLight® 594 conjugated used at a dilution of 1:100.