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Rabbit anti-Phospho SMC1 (S966) Antibody, Affinity Purified

Catalogue number:
A300-050A
Size:
100 µg
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Antibody was affinity purified using an epitope specific to phosphorylation of SMC1 at serine 966 immobilized on solid support. Immunogen for A300-050A was a phosphorylated synthetic peptide, which represented a portion of human Structural Maintenance of Chromosomes 1 (GeneID 8243) around serine 966 according to the numbering given in entry NP_006297.2. Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.

  • Applications: WB, IP, IHC, ICC-IF
Product Type:

Antibodies

Alternative Names:

CDLS2, DXS423E, epididymis secretory sperm binding protein, SB1.8, segregation of mitotic chromosomes 1, SMC protein 1A, SMC1, SMC1 (structural maintenance of chromosomes 1, yeast)-like 1, SMC-1A, SMC1alpha, SMC-1-alpha, SMC1L1, SMCB, structural maintenan

Species Reactivity:

Human

Immunogen:

surrounding serine 966

Storage Temp:

2 - 8°C

Antibody Host:

Rabbit

Antibody Isotype:

Whole IgG

Antibody Type:

Polyclonal

UNSPSC Code:

12352203

Detection of Phospho SMC1 (S966) by west

Detection of Phospho SMC1 (S966) by western blot. Samples: Whole cell lysate (50 µg) from HeLa cells that were treated with NCS (+; 200 ng/ml; 1h) or mock treated (-). Antibodies: Affinity purified rabbit anti-Phospho SMC1 (S966) antibody A300-050A (lot A300-050A-6) used at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes. For detection of total SMC1, rabbit anti-SMC1 antibody A300-055A was used.

Detection of human Phospho SMC1 (S966) b

Detection of human Phospho SMC1 (S966) by immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti- Phospho SMC1 (S966) (Cat. No. A300-050A Lot6) used at a dilution of 1:1,000 (1µg/ml). Detection: DAB

Detection of human Phospho SMC1 (S966) b

Detection of human Phospho SMC1 (S966) by immunocytochemistry. Samples: Neocarzinostatin treated asynchronous HeLa cells. Antibody: Affinity purified rabbit anti-Phospho SMC1 (S966) (Cat. No. A300-050 Lot6) used at a dilution of 1:5,000 (0.2µg/ml). Detection: Red fluorescent Anti-rabbit IgG-DyLight® 594 conjugated used at a dilution of 1:100.