Antibody was affinity purified using an epitope specific to phosphorylation of SMC1 at serine 957 immobilized on solid support. Immunogen for A300-045A was a phosphorylated synthetic peptide, which represented a portion of human Structural Maintenance of Chromosomes 1 (GeneID 8243) around serine 957 according to the numbering given in entry NP_006297.2. Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
CDLS2, DXS423E, epididymis secretory sperm binding protein, SB1.8, segregation of mitotic chromosomes 1, SMC protein 1A, SMC1, SMC1 (structural maintenance of chromosomes 1, yeast)-like 1, SMC-1A, SMC1alpha, SMC-1-alpha, SMC1L1, SMCB, structural maintenan
surrounding serine 957
2 - 8°C
Detection of human Phospho SMC1 (S957) by western blot. Samples: Whole cell lysate (50 µg) from HEK293T cells treated with 100 µM etoposide for 4 hours (+) or mock treated (-) cells. Antibody: Affinity purified rabbit anti-Phospho SMC1 (S957) antibody A300-045A (lot A300-045A-3) used for WB at 0.5 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.