Rabbit anti-DNMT1 Antibody, Affinity Purified
Antibody was affinity purified using an epitope specific to DNMT1 immobilized on solid support. The epitope recognized by A300-042A maps to a region between residue 700 and 750 of human DNA-Methyltranserase 1 using the numbering given in entry NP_001370.1 (GeneID 1786). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
Antibodies
ADCADN, AIM, CXXC9, CXXC-type zinc finger protein 9, DNA (cytosine-5-)-methyltransferase 1, DNA (cytosine-5)-methyltransferase 1, DNA methyltransferase HsaI, DNA MTase HsaI, DNMT, Dnmt1, HSN1E, m.HsaI, MCMT
Human
between 700 and 750
2 - 8°C
Rabbit
Whole IgG
Polyclonal
12352203
Detection of human DNMT1 by western blot. Samples: Whole cell lysate (50 µg) from HeLa and HEK293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-DNMT1 antibody A300-042A (lot A300-042A-2) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Detection of human DNMT1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-DNMT1 antibody A300-042A (lot A300-042A-2) used for IP at 3 µg per reaction. For blotting immunoprecipitated DNMT1, A300-042A was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.