Rabbit anti-DNMT1 Antibody, Affinity Purified - A300-042A - 100 µg - Bethyl Laboratories, Inc. - antibodies
Antibody was affinity purified using an epitope specific to DNMT1 immobilized on solid support. The epitope recognized by A300-042A maps to a region between residue 700 and 750 of human DNA-Methyltranserase 1 using the numbering given in entry NP_001370.1 (GeneID 1786). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
Applications:
WB, IP
Detection of human DNMT1 by western blot. Samples: Whole cell lysate (50 µg) from HeLa and HEK293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-DNMT1 antibody A300-042A (lot A300-042A-2) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Detection of human DNMT1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-DNMT1 antibody A300-042A (lot A300-042A-2) used for IP at 3 µg per reaction. For blotting immunoprecipitated DNMT1, A300-042A was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
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