Antibody was affinity purified using an epitope specific to RENT1 immobilized on solid support. The epitope recognized by A300-038A maps to a region between residue 1075 and the C-terminus (residue 1118) of human Regulator of Nonsense Transcripts 1 (up-frameshift mutation 1 homolog) using the numbering given in entry NP_002902.2 (GeneID 5976). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
ATP-dependent helicase RENT1, delta helicase, HUPF1, nonsense mRNA reducing factor 1, NORF1, pNORF1, regulator of nonsense transcripts 1, RENT1, smg-2, smg-2 homolog, nonsense mediated mRNA decay factor, UPF1 regulator of nonsense transcripts homolog, up-
between 1075 and C-term
2 - 8°C
Detection of human RENT1 by western blot. Samples: Whole cell lysate (50 µg) from HEK293T, RKO, MCF-7, Jurkat, TMCK-1, and NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-RENT1 antibody A300-038A (A300-038A lot 4) used at 0.04 µg/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.
Detection of human RENT1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-RENT1 antibody A300-038A (A300-038A lot 4) used for IP at 6 µg per reaction. RENT1 was also immunoprecipitated by a previous lot of this antibody (lot A300-038A-3). For blotting immunoprecipitated RENT1, A300-038A was used at 0.04 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.