Antibody was affinity purified using an epitope specific to CSN1 immobilized on solid support. The epitope recognized by A300-026A maps to a region between residue 400 and the C-terminus (residue 471) of human G protein Pathway Suppressor 1 using the numbering given in Swiss-Prot entry Q13098 (GeneID 2873). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
COP9 signalosome complex subunit 1, COPS1, CSN1, G protein pathway suppressor 1, GPS-1, JAB1-containing signalosome subunit 1, Protein MFH, SGN1, Signalosome subunit 1
between 400 and C-term
2 - 8°C
Detection of human CSN1 by western blot. Samples: Whole cell lysate (50 µg) from HeLa and HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-CSN1 antibody A300-026A (lot A300-026A-1) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Detection of human CSN1 by immunohistochemistry. Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti-CSN1 (Cat. No. A300-026A) used at a dilution of 1:1,000 (1µg/ml). Detection: DAB
Detection of human CSN1 by western blot. Samples: Whole cell lysate (50 µg - E; 10 µg - T) from mock transfected (E) or CSN1 transfected (T) HEKHEK293T cells. Antibody: Affinity purified rabbit anti-CSN1 A300-026A used at the indicated concentration. Detection: Chemiluminescence with a 10 second exposure.