Antibody was affinity purified using an epitope specific to CSN5 immobilized on solid support. The epitope recognized by A300-014A maps to a region between residues 175 and 225 of human COP9 constitutive photomorphogenic homolog subunit 5 using the numbering given in entry NP_006828.2 (GeneID 10987). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
38 kDa Mov34 homolog, COP9 constitutive photomorphogenic homolog subunit 5, COP9 signalosome complex subunit 5, CSN5, JAB1, jun activation domain-binding protein 1, MOV-34, SGN5, signalosome subunit 5, testis secretory sperm-binding protein Li 231m
between 175 and 225
2 - 8°C
Detection of human CSN5 by western blot. Samples: Whole cell lysate (50 µg) from HeLa and HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-CSN5 antibody A300-014A (lot A300-014A-1) used for WB at 0.2 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.
Detection of human CSN5 by western blot. Samples: Whole cell lysate (50 µg - E; 25 µg - T) from HEK HEK293T cells that were mock transfected (E) or transfected with a CSN5 expression construct (T).Antibody: Affinity purified rabbit anti-CSN5 antibody A300-014A used at the indicated concentrations. Detection: Chemiluminescence with a 10 second exposure.