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Rabbit anti-AU5 Tag Antibody Affinity Purified

Catalogue number:
A190-227A
Size:
1 mg
Product is available in:
  • UK
  • Ireland
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£854.00
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Rabbits were immunized with AU5 (TDFYLK) conjugated to KLH. Antibody was isolated by affinity chromatography using the peptide immobilized on solid support. Antibody concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.

  • Applications: WB, IP, ICC, ELISA
Product Type:

Antibodies

Storage Temp:

2 - 8°C

Antibody Host:

Rabbit

Antibody Isotype:

Whole IgG

Antibody Type:

Polyclonal

UNSPSC Code:

12352203

Detection of AU5-tagged Protein by weste

Detection of AU5-tagged Protein by western blot. Samples: 200, 100, or 50 ng of E. coli whole cell lysate expressing a multi-tag fusion protein. Antibodies: Affinity purified, rabbit anti-AU5 antibody A190-227A used for WB at 0.2 µg/ml (1:5,000). Detection: Chemiluminescence with an exposure time of 3 seconds.