Goat anti-Myc Tag Antibody Affinity Purified - A190-204A - 1 mg - Bethyl Laboratories, Inc. - antibodies
Goats were immunized with a synthetic peptide representing amino acid residues 410-419 (EQKLISEEDL) of human myc conjugated to KLH. Antibody was isolated by affinity chromatography using the peptide immobilized on solid support. Antibody concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
Applications:
WB, IP, ICC, ELISA
Detection of c-myc-tagged Protein by western blot. Samples: 200, 100, or 50 ng of E. coli whole cell lysate expressing a multi-tag fusion protein. Antibodies: Affinity purified, goat anti-c-myc antibody A190-204A used for WB at 0.04 µg/ml (1:25,000). Detection: Chemiluminescence with an exposure time of 30 seconds.
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