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Goat anti-GST Tag Antibody HRP Conjugated

Catalogue number:
A190-121P
Size:
Each
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Goats were immunized with Glutathione-S-Transferase (GST) from Schistosoma japonicum. Antibody was isolated by affinity chromatography using GST immobilized on solid support and conjugated to horseradish peroxidase (HRP). Antibody concentration was determined by extinction coefficient prior to conjugation: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG. Molar enzyme/antibody protein ratio is 4:1.

Product Type:

Antibodies

Storage Temp:

2 - 8°C

Antibody Host:

Goat

Antibody Isotype:

Whole IgG

Formulation:

Phosphate Buffered Saline (PBS) containing 0.2% BSA and 0.05% Pro-Clean 400

Antibody Type:

Polyclonal

UNSPSC Code:

12352203

Detection of GST-tagged Protein by weste

Detection of GST-tagged Protein by western blot. Samples: 0.1, 0.3, or 1.0 µg of 293 cell lysate expressing a GST-tag fusion protein. Antibodies: Affinity-purified, HRP-conjugated, goat anti-GST antibody A190-121P used for WB at 0.06 µg/ml (1:15,000). Detection: Chemiluminescence with an exposure time of 30 seconds.