Goat anti-GST Tag Antibody Biotinylated

Catalogue number:

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A190-121B

Supplier:

Bethyl Laboratories, Inc.

Size:

0.1 mg _$$_

Product is available in:

N/A

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Applications:

WB, ELISA

Antibody Host:

Goat

Antibody Isotype:

Whole IgG

Antibody Type:

Polyclonal

Data sheet: View or download
MSDS: View or download

Product Description:

Goats were immunized with Glutathione-S-Transferase (GST) from Schistosoma japonicum. Antibody was isolated by affinity chromatography using GST immobilized on solid support and conjugated to biotin. Antibody concentration was determined by extinction coefficient prior to conjugation: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.

Storage Temperature:

2 - 8°C

Detection of GST-tagged Protein by western blot. Samples: 0.1, 0.3, or 1.0 µg of 293 cell lysate expressing a GST-tag fusion protein. Antibodies: Affinity-purified, biotin-conjugated, goat anti-GST antibody A190-121B used for WB at 0.06 µg/ml (1:15,000). Detection: Streptavidin-HRP (0.1 µg/ml) and chemiluminescence with an exposure time of 60 seconds.

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