Goat anti-GST Antibody Biotinylated - A190-121B - 0.1 mg - Bethyl Laboratories, Inc. - antibodies
Goats were immunized with Glutathione-S-Transferase (GST) from Schistosoma japonicum. Antibody was isolated by affinity chromatography using GST immobilized on solid support and conjugated to biotin.Antibody concentration was determined by extinction coefficient prior to conjugation: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
Applications:
WB, ELISA
Detection of GST-tagged Protein by western blot. Samples: 0.1, 0.3, or 1.0 µg of 293 cell lysate expressing a GST-tag fusion protein. Antibodies: Affinity-purified, biotin-conjugated, goat anti-GST antibody A190-121B used for WB at 0.06 µg/ml (1:15,000). Detection: Streptavidin-HRP (0.1 µg/ml) and chemiluminescence with an exposure time of 60 seconds.
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