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Goat anti-GST Tag Antibody Biotinylated

Catalogue number:
A190-121B
Size:
0.1 mg
Product is available in:
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Goats were immunized with Glutathione-S-Transferase (GST) from Schistosoma japonicum. Antibody was isolated by affinity chromatography using GST immobilized on solid support and conjugated to biotin. Antibody concentration was determined by extinction coefficient prior to conjugation: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.

  • Applications: WB, ELISA
Detection of GST-tagged Protein by weste
Product Type:

Antibodies

Storage Temp:

2 - 8°C

Antibody Host:

Goat

Antibody Isotype:

Whole IgG

Antibody Type:

Polyclonal

UNSPSC Code:

12352203

Detection of GST-tagged Protein by weste

Detection of GST-tagged Protein by western blot. Samples: 0.1, 0.3, or 1.0 µg of 293 cell lysate expressing a GST-tag fusion protein. Antibodies: Affinity-purified, biotin-conjugated, goat anti-GST antibody A190-121B used for WB at 0.06 µg/ml (1:15,000). Detection: Streptavidin-HRP (0.1 µg/ml) and chemiluminescence with an exposure time of 60 seconds.