Goats were immunized with purified IgG from dove, duck, sparrow and chicken serum. The antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to horseradish peroxidase (HRP). Antibody concentration was determined by extinction coefficient prior to conjugation: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG. Molar enzyme/antibody protein ratio is 4:1. By immunoelectrophoresis and ELISA this antibody reacts with dove, duck, sparrow and chicken, turkey, emu, ostrich, quail, macaw and cockateil IgG. No antibody was detected against non-immunoglobulin serum proteins. This antibody may cross react with IgG from other species.
- Applications: WB, IHC, ICC, ELISA
2 - 8°C