Antiserum was solid phase adsorbed to ensure specificity. The antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to horseradish peroxidase (HRP). Antibody concentration was determined by extinction coefficient prior to conjugation: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG. Molar enzyme/antibody protein ratio is 4:1. By immunoelectrophoresis and ELISA this antibody reacts specifically with Bovine alpha-Lactalbumin. This antibody may cross react with alpha lactalbumin from other species.
2 - 8°C
Phosphate Buffered Saline (PBS) containing 0.2% BSA and 0.05% Pro-Clean 400