Antiserum was solid phase adsorbed to ensure subclass specificity. The antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to alkaline phosphatase (alkphos). Antibody concentration was determined by extinction coefficient prior to conjugation: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG. Molar enzyme:antibody protein ratio is 1:1. By immunoelectrophoresis and ELISA this antibody reacts specifically with bovine IgG1. This antibody may cross react with IgG1 from other species.
2 - 8°C
50 mM HEPES pH 7.1, 0.1 M NaCl, 1 mM MgCl2, 0.1 mM ZnCl2 containing 0.09% NaN3