Excellent reagent for detecting polyhistidine-containing biomolecules
Reagents & Kit Components
-20°C desiccated and protected from light
McMahan SA and Burgess RR (1996). Single-step synthesis and characterization of biotinylated nitrilotriacetic acid, a unique reagent for the detection of histidine-tagged proteins immobilized on nitrocellulose. Anal Biochem 236, 101-6; O''Shannessy DJ, et al. (1995). Detection and quantitation of hexa-histidine-tagged recombinant proteins on western blots and by a surface plasmon resonance biosensor technique. Anal Biochem 229, 119-24.