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Rabbit anti-GR Antibody, Affinity Purified

Catalogue number:
A303-491A
Size:
20 µg
Product is available in:
  • UK
  • Ireland
  • Europe
  • USA
  • Rest of World
£383.00 Estimated delivery Wed 26 Jan
Shipping is calculated in checkout

Rabbit anti-GR Antibody, Affinity Purified - 20 µg

  • Applications: WB, IP, IHC, ChIP-Seq
Product Type:

Antibodies

Alternative Names:

GCCR, GCR, GCRST, glucocorticoid nuclear receptor variant 1, glucocorticoid receptor, GR, GRL, Nuclear receptor subfamily 3 group C member 1, nuclear receptor subfamily 3, group C, member 1 (glucocorticoid receptor)

Species Reactivity:

Human

Immunogen:

between 150 and 200

Storage Temp:

2 - 8°C

Antibody Host:

Rabbit

Antibody Isotype:

Whole IgG

Antibody Type:

Polyclonal

UNSPSC Code:

12352203

Detection of human GR by western blot an

Detection of human GR by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded) and HEK293T (T; 50 µg) cells. Antibodies: Affinity purified rabbit anti-GR antibody A303-491A used for WB at 0.04 µg/ml (A) and 0.4 µg/ml (B) and used for IP at 6 µg/mg lysate. GR was also immunoprecipitated by rabbit anti-GR antibody A303-490A, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 1 second (B).

Detection of human GR by immunohistochem

Detection of human GR by immunohistochemistry. Sample: FFPE section of human lung carcinoma. Antibody: Affinity purified rabbit anti- GR (Cat. No. A303-491A Lot1) used at a dilution of 1:200 (1µg/ml). Detection: DAB

Localization of GR Binding Sites by ChIP

Localization of GR Binding Sites by ChIP-sequencing. Chromatin from prednisolone treated THP-1 cells was immunoprecipitated with anti-GR antibody A303-491A and analyzed by DNA sequencing. The figure illustrates the peak distribution of GR binding within a 250 Kb region of chromosome 6 as detected using anti-GR A303-491A. ChIP-seq validation performed by Active Motif, Carlsbad, CA.

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