C1orf39, formin-binding protein 1-like, TOCA1, toca-1, transducer of Cdc42-dependent actin assembly 1, transducer of Cdc42-dependent actin assembly protein 1
between 450 and 500
2 - 8°C
Detection of human Toca-1 by western blot and immunoprecipitation. Samples: Whole cell lysate from Jurkat (15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded), HEK293T (T; 50 µg) and HeLa (H; 50 µg) cells. Antibodies: Affinity purified rabbit anti-Toca-1 antibody A303-470A used for WB at 0.1 µg/ml (A) and 1 µg/ml (B) and used for IP at 6 µg/mg lysate. Toca-1 was also immunoprecipitated by rabbit anti-Toca-1 antibody A303-469A, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 30 seconds (B).